Monitoring T cell-mediated immunity is crucial for assessing the immune status in the context of SARS-CoV-2 infection and vaccination.
Immune Analytics supports your (pre)clinical studies or research projects with a highly sensitive and specific SARS-CoV-2 FluoroSpot assay (IFN-γ / IL-2) utilizing our proprietary SARS-CoV-2 specific antigen pool. If your project requires detection of additional / other cytokines or a different cytokine pool, we can easily adapt the assay to your specific requirements.
Our quality management system meets the highest standards concerning the quality and integrity of all data we produce and ensures due diligence with regard to sample management, data generation and analysis, documentation and archiving and clinical trial subject rights.
T cell reactivity against SARS-CoV-2 antigens analyzed by FluoroSpot.
The SARS-COV-2 FluoroSpot results are visualized as Tukey box plots of serologically negative (n = 48) and positive donors (n = 100).
Stimulation indexes (Sls; spot count antigen stimulated sample divided by spot count unstimulated sample) of IFN-y, IL-2 and IFN-y + IL-2 T cell response against SARS-CoV-2 antigens indicate a highly significant difference (**; P value <0.0001) between both groups. P values are calculated by the unpaired, parametric t-test.
The solid line indicates the median, the box indicates the interquartile range (the difference between the 25th and 75th percentiles), the whiskers indicate the range excluding “outliers” and the points indicate outliers, points more than 1.5 IQR from the box.
ROC curve: SARS-CoV-2 IFN-y / IL-2 FluoroSpot
The receiver operating characteristic (ROC) curve shows the true positive rate (sensitivity) versus the false positive rate (100-specificity) of SARS-CoV-2 IFN-γ / IL-2 FluoroSpot for different cut-offs.
Results were compiled by interrogating clinical diagnostics (PCR and serology).
T cell reactivity to SARS-CoV-2 vaccines.
SARS-Cov-2 FluoroSpot results (SI; spot count antigen stimulated sample divided by spot count unstimulated sample) before vaccination (TO), two weeks after the first vaccination (T1) and two weeks after the second vaccination (T2) of 11 donors are visualized as box plots.
Data points obtained from each donor are connected by lines.